Cite this article as:

Romanova E. B., Nikolaev V. Y., Bakiev A. G., Klenina A. A. LEUKOCYTE BLOOD COMPOSITION FEATURES OF GRASS SNAKE (NATRIX NATRIX) AND DICE SNAKE (N. TESSELLATA) (REPTILIA: COLUBRIDAE) FEMALES IN THE SAMARA REGION. Izvestiya of Saratov University. New series. Series: Earth Sciences, 2015, vol. 15, iss. 1, pp. 69-?.


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574.3.591

LEUKOCYTE BLOOD COMPOSITION FEATURES OF GRASS SNAKE (NATRIX NATRIX) AND DICE SNAKE (N. TESSELLATA) (REPTILIA: COLUBRIDAE) FEMALES IN THE SAMARA REGION

Abstract

Our study of the peripheral blood leukogram of grass snake and dice snake females (Samara region) has revealed changes in the blood of these snakes before and after their laying eggs, associated with the natural immunity activation in these animals. Natural, innate immunity reactions were mediated by mononuclear cells (monocytes and lymphocytes). An increase in the lymphocyte fraction in the dice snake female blood is shown to reduce the leukogram shift index. Interspecific differences were revealed, manifested themselves as a significant increase in the monocyte fraction in the peripheral blood of dice snake females as compared with grass snake females after laying eggs.

References

Воробьева А. С. 2001. Сравнительная характе-ристика периферической крови змей Волжского бассейна // Актуальные проблемы герпетологии и токсикологии / Ин- экологии Волжского бассейна РАН. Тольятти. Вып. 10. С. 25 – 30.
Житенева Л Д., Рудницкая О. А., Калюжная Т. И. 1997. Эколого-гематологические характеристики некоторых видов рыб : справочник. Ростов н/Д : АзНИИРХ. 149 с.
Лисничая Е. Н., Ефимов В. Г. 2014. Особенности исследования морфологического состава крови рептилий // Науково-техничний бюлетень НДЦ бiобезпеки та екологiчного контролю ресурсiв АПК. Т. 2, № 1. С. 61 – 74. 
Реброва О. Ю. 2006. Статистический анализ медицинских данных. Применение пакета прикладных программ STATISTICA. М. : МедиаСфера. 312 с.
Романова Е. Б., Николаев В. Ю., Бакиев А. Г., Клёнина А. А. 2014. Лейкоцитарный состав крови обыкновенного ужа (Natrix natrix) и водяного ужа (N. tessellata) из Национального парка «Самарская Лука» // Изв. Самар. науч. центра РАН. Т. 16, № 1. С. 152 – 155. 
Соколина Ф. М., Павлов А. В., Юсупов Р. Х. 1997. Гематология пресмыкающихся : метод. пособие к курсу герпетологии, большому практикуму и семинарам. Казань : Изд-во Казан. ун-та. 31 с.
Хайрутдинов И. З., Павлов А. В., Соколина Ф. М. 2008. Сравнительная морфология крови двух видов рептилий // Вопросы герпетологии : материалы Третьего съезда Герпетол. о-ва им. А. М. Никольского / Зоол. ин-т РАН. СПб. С. 415 – 422.
Хайрутдинов И. З., Соколина Ф. М. 2010. Характеристика крови рептилий и ее связь с условиями среды обитания : учеб.-метод. пособие к курсу «Герпетология». Казань : Изд-во Казан. ун-та. 44 с.
Arican H., Cicek K. 2014. Haematology of amphibians and reptiles : a review // North-Western J. of Zoology. Vol. 10, № 1. P. 190 – 209. 
Coico R., Sunshine G., Benjamini E. 2003. Immunology. A Short Course. Hoboken : Wiley-Liss Publications. 500 p. 
Kakizoe Y., Sakaoka K., Kakizoe F. 2007. Successive changes of hematologic characteristics and plasma chemistry values of juvenile loggerhead turtles (Caretta caretta) // J. of Zoo and Wildlife Medicine. Vol. 38, № 1. P. 77 – 84.
Li J., Barreda D. R., Zhang Y. A., Boshra H., Gelman A. E., Lapatra S., Tort L., Sunyer J. O. 2006. B lymphocytes from early vertebrates have potent phagocytic and microbiological abilities // Nature Immunology. Vol. 7, № 10. P. 1116 – 1124. 
Martin L. B. 2009. Stress and immunity in wild vertebrates : timing is everything // General and Comparative Endocrinology. Vol. 163, № 1. P. 70 – 76.
Martin L. B., Weil Z. M., Nelson R. J. 2008. Seasonal changes in vertebrate immune activity : mediation by physiological trade-offs // Philosophical Transactions of the Royal Society of London. Series B. Biological Sciences. Vol. 363. P. 321 – 339.
Mondal S., Rai U. 2002. In vitro effect of sex steroids on cytotoxic activity of splenic macrophages in wall lizard (Hemidactylus flaviviridis) // General and Comparative Endocrinology. Vol. 125, № 2. P. 264 – 271. 
Montali R. J. 1988. Comparative pathology of in-flammation in the higher vertebrates (Reptiles, birds and mammals) // J. Comparative Pathology. Vol. 99, № 1. P. 1 – 26. 
Oros J., Casal A. B., Arencibia A. 2010. Microscopic studies on characterization of blood cells of endangered sea turtles // Microscopy : Science, Technology, Applications and Education. Vol. 1. P. 75 – 84.
Saad A. H. 1989. Sex-associated differences in the mitogenic responsiveness of snake blood lymphocytes // Developmental and Comparative Immunology. Vol. 13, № 3. P. 225 – 229.
Salem M. L. 2004. Estrogen, a doubleedged sword : modulation of TH1-and TH2-mediated inflammations by differential regulation of TH1/TH2 cytokine production // Current Drug Targets. Inflammation and Al-lergy. Vol. 3, № 1. P. 97 – 104.
Scapigliati G., Buonocore F., Mazzini M. 2006. Biological activity of cytokines : an evolutionary perspec-tive // Current Pharmaceutical Design. Vol. 12, № 24. P. 3071 – 3081.
Stacy N. I., Alleman A. R., Sayler K. A. 2011. Di-agnostic hematology of Reptiles // Clinics in Laboratory Medicine. Vol. 31, iss. 1. P. 87 – 108.
Stahl S. J. 2006. Reptile hematology and serum chemistry // Proc. of the North American Veterinary Conference. Vol. 20. P. 1673 – 1676.
Sypek J. P., Borysenko M., Findlay S. R. 1984. Antiimmunoglobulin induced histamine release from naturally abundant basophils in the snapping turtle, Che-lydra serpentina // Developmental and Comparative Immunology. Vol. 8, № 2. P. 359 – 366. 
Tavares-Dias M., Oliveira-Junior A. A., Marcon J. L. 2008. Methodological limitations of counting total leuko-cytes and thrombocytes in reptiles (Amazon turtle, Podocnemis expansa) : an analysis and discussion // Acta Amazonica. Vol. 38, № 2. P. 351 – 356.
Zhang F. 2010. A Review of Chelonian Hematology // Asian Herpetology Research. Vol. 2, № 1. P. 12 – 20.
Zhou X., Guo Q., Dai H. 2009. Molecular characterization and expression profiles in response to bacterial infection of Chinese soft-shelled turtle interleukin-8 (IL-8), the first reptilian chemokine gene // Developmental and Comparative Immunology. Vol. 33, № 7. P. 838 – 847. 
Zimmerman L. M., Vogel L. A., Bowden R. M. 2010. Understanding the vertebrate immune system: in-sights from the reptilian perspective // J. of Experimental Biology. Vol. 213. P. 661 – 671.

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