This study presents the results of our searching for antimicrobial peptide genes in the amphibian skin granular glands using next-generation sequencing. Bioinformatic analysis of genomic data from 36 individuals belonging to 10 species of anuran amphibians revealed sequences of esculentin, temporin, ranacyclin, bradykinin (kininogen-1), and bombinin in three amphibian species (Bombina bombina, Rana arvalis, and Pelophylax ridibundus).