This study aimed to clarify the nature and extent of the contact zone between subspecies of the sand lizard Lacerta agilis chersonensis and L. a. exigua in the Central Chernozem Region. Based on mitochondrial DNA (mtDNA) marker analysis, the boundary of their ranges in the studied area is located in the western part of the Kursk region, specifically within the Solntsevsky and Manturovsky districts. No mixed populations were found; the distance between the closest localities of the eastern and southern subspecies was 16 km.
