For the first time, the amplitude-frequency and temporal characteristics of the acoustic signals (the distress one and two types of advertisement calls) of the Crimean gecko (Mediodactylus danilewskii) were analyzed. The distress call is a rather long one (usually around 150–350 ms, but sometimes up to nearly 1 s), with a peak frequency of 6.86 kHz in both males and females. The upper harmonics of this type of signal lie in the ultrasonic region of the frequency spectrum.