The paper presents data on the variability of basic morphological characters of the sand lizard (Lacerta agilis) inhabiting the vicinities of the cities Tyumen (Western Siberia) and Sterlitamak (Southern Urals). Five subtypes of back coloration were identified. Typical and atypical variants of the coloration pattern are described. A high level of the genetic variability of the ISSR markers of sand lizards was revealed. It was significantly lower in the group of mature individuals as compared to juveniles.