hypothermia

Storage of grass frog Rana temporaria (Ranidae, Amphibia) oocytes in a carbon monoxide and oxygen mixture under pressure

The development of a technology for hypothermic preservation of amphibian oocytes is one of the urgent tasks for cryobiology. The previously proposed method of “dry” hypothermic preservation in small sealed containers allowed us to increase the duration of refrigerated storage of unfertilized oocytes of the grass frog Rana temporaria up to 7 days (fertilization 40%; hatching 20%).

On the issue of the thermal adaptation of the larvae caucasian brown frog Rаna macrocnemis Boulenger, 1885 (Amphibia, Ranidae) to low-temperature environmental conditions

The results of the study of hematological parameters of the blood of the larvae caucasian brown frog Rana macrocnemis and the total activity of catalase compared with adults and at different temperature conditions: at 23°C and after five days of hypothermia at 5°C. Differences in the blood formula were revealed in different periods of ontogenesis and under the influence of artificial hypothermia.