reproductive technologies

New Approaches to Collecting Reproductive Material from Amphibians for its Use in Artificial Fertilization

This paper describes new methods for obtaining viable ovulated oocytes and testicular sperm from the carcasses of females and males of the common frog Rana temporaria, stored at +4ºС for 1–7 days. In addition, a new approach to delayed collection (1 to 30 days) of ovulated oocytes from live female frogs of the same species is given. Part of the frog testicular spermatozoa is shown to retain motility (21.0±1.5%) and fertilizing ability (13.2±1.9%) even after 6 days of storage at +4°C in the carcasses of males.