We analyzed the karyotypes of the most toad species of the family Bufonidae inhabiting the Palearctic (6 genera, 29 species). All diploid species had 11 chromosome pairs. In most of them (Bufo, Bufotes and Barbarophryne) the nucleolar organizer (NOR) was located on the 6th pair and only in few species of the genus Bufotes on the 5th one, Strauchbufo – on the 4th, Epidalea – on the 11th, and Sclerophrys – on the 10th one, respectively.