The article provides information about the history of the formation of the memorial collection of amphibians and reptiles obtained by the Zoological Museum of Moscow University from the Godeffroy Trade Museum at the end of the XIX century. The collection includes 20 specimens of 16 amphibian species and 86 specimens of 70 reptile species. A complete list of collection samples, their taxonomic and geographical analysis is given. Most of the amphibians in the Godeffroy collection belong to the Australian tree frogs (Hylidae: Pelodryadinae).