We investigated seasonal changes in the plasma levels of sex hormones in males (testosterone) and females (progesterone and estradiol) of Elaphe dione (Pallas 1773). Snakes (21 males and 17 females) were captured from May to August 2022 on the Samarskaya Luka (Samara Region, Russian Federation). Some individuals (4 males and 11 females) after capture were kept under controlled laboratory conditions for two months, then they were released at the capture sites. Blood was sampled immediately after capture.