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Romanova E. B., Bakiev A. G., Gorelov R. A. Adaptive blood reactions of Testudo graeca nikolskii Ckhikvadze et Tuniyev, 1986 (Testudinidae, Reptilia). Current Studies in Herpetology, 2024, vol. 24, iss. 3, pp. 163-170. DOI: https://doi.org/10.18500/1814-6090-2024-24-3-4-163-170
Adaptive blood reactions of Testudo graeca nikolskii Ckhikvadze et Tuniyev, 1986 (Testudinidae, Reptilia)
In order to check the health of individuals of the Mediterranean tortoise Nikolskii Testudo graeca nikolskii Ckhikvadze et Tuniyev, 1986 (22 individuals: 6 males and 16 females) collected in the vicinity of Anapa (Krasnodar region of Russia), a haematological approach was used to assess adaptive blood reactions. Intraerythrocytic parasites Haemogregarina spp. (Adeleorina, Coccidia) were detected in blood smears of 54.5% of turtles. Female were more prone to infection with hemogrega-rines than males, as evidenced by higher invasion index, mean invasion intensity and proportion of infected cells. Individual stages of hemoparasite development (gametocytes and trophozoites) were found in blood erythrocytes with equal frequency. No differences were found in the leukocyte composition of the blood between infected and healthy female and male. Lymphocytes predominated in the leukogram of turtles. The dependence of the increase in the number of eosinophils in the peripheral blood of turtles on the content of trophozoites in erythrocytes was revealed. Parasitic forms of Haemogregarina spp. affected the host's immune response and it is necessary to identify possible harmful long-term consequences of infection for T. g. nikolskii Ckhikvadze et Tuniyev, 1986 from Krasnodar region.
Red Book of the Krasnodar Region. Animals. 3 edition. Krasnodar, Krasnodar Krai Administration Publ., 2017. 720 p. (in Russian).
Red Book of the Russian Federation. Vol. Animals. 2nd edition. Moscow, FGBU “VNII Ecology” Publ., 2021. 1128 p. (in Russian).
Malyutina T. A. Mutual relations in the parasite-host system: Biochemical and physiological aspects of adaptation (retrospective review). Russian Parasitological Journal, 2008, no. 1, pp. 1–17 (in Russian).
Sokolina F. M., Pavlov A. V., Yusupov R. Kh. Gematologiya presmykayushchikhsya. Metodicheskoe posobie po kursu “Gerpetologiya”, bol'shomu praktikumu i spetsseminaram [Hematology of Reptiles. Methodological Manual for the Course of Herpetology, a Large Workshop and Special Seminars]. Kazan, Kazan State University Publ., 1997. 31 p. (in Russian).
Chkhikvadze V. M., Tuniev B. S. On the systematic position of the modern land tortoise of Western Transcaucasia. Reports of the Academy of Sciences of the Georgian SSR, 1986, vol. 124, no. 3, pp. 617–620 (in Russian).
Shevkoplyas V. N., Lopatin V. G. Influence of helminthoses on the course of immunological processes in animals. Russian Journal of Parasitology, 2008, no. 4, pp. 94–101 (in Russian).
Adl S. M., Simpson A. G. B., Lane C. E., Lukeš J., Bass D., Bowser S. S., Brown M. W., Burki F., Dunthorn M., Hampl V., Heiss A., Hoppenrath M., Lara E., le Gall L., Lynn D. H., McManus H., Mitchell E. A. D., Mozley-Stanridge S. E., Parfrey L. W., Pawlowski J., Rueckert S., Shadwick L., Schoch C. L., Smirnov A., Spiegel F. W. The revised classification of eukaryotes. Journal of Eukaryotic Microbiology, 2012, vol. 59, iss. 5, pp. 429–514. https://doi.org/10.1111/j.1550-7408.2012.00644.x
Alleman A. R., Jacobson E. R., Raskin R. E. Morphologic and cytochemical characteristics of blood cells from the desert tortoise (Gopherus agassizii). American Journal of Veterinary Research, 1992, vol. 53, pp. 1645–1651.
International Guiding Principles for Biomedical Research Involving Animals. Geneva, Council for International Organization of Medical Sciences Publ., 2012. 4 p.
Kirsche W. Die Landschildkröten Europas. Biologie, Pflege, Zucht und Schutz. Melle, Mergus Verlag GmbH., 1998. 104 S.
Mihalca A., Achelaritei D., Popescu P. Haemoparasites of the genus Haemogregarina in a population of European pond turtles (Emys orbicularis) from Drăgăşani, Valcea county, Romania. Scientia Parasitologica, 2002, vol. 2, pp. 22–27.
Mihalca A., Racka K., Gherman C., Ionescu D. T. Prevalence and intensity of blood apicomplexan infections in reptiles from Romania. Parasitology Research, 2008, vol. 102, iss. 5, pp. 1081–1083. https://doi.org/10.1007/ s00436-008-0912-9
Salakij C., Salakij J., Suthunmapinunta P., Chanhome L. Hematology, morphology and ultrastructure of blood cells and blood parasites from puff-faced watersnakes (Homalopsis buccata). Kasetsart Journal – Natural Science, 2002, vol. 36, pp. 35–43.
Siddall M. E., Desser S. S. Transmission of Haemogregarina balli from painted turtles to snapping turtles through the leech Placobdella ornate. Journal of Parasitology, 2001, vol. 87, iss. 5, pp. 1217–1218. https://doi.org/10.1645/0022-3395(2001)087[1217:TOHBFP]2.0.CO;2
Telford Jr. S. R. Hemoparasites of the Reptilia: Color Atlas and Text. New York, CRC Press, 2008. 376 p.
Uetz P., Freed P., Aguilar R., Reyes F., Kudera J., Hošek J., eds. The Reptile Database, 2024. Available at: https:// www.reptile-database.org (accessed May 22, 2024).
